Repeated cycles of amplification cause degradation of your probe, resulting in amplified fluorescence. TaqMan detection is, thus, extra particular than dye‐dependent detection, because an increase in fluorescence occurs only upon binding of the probe to a specific sequence and subsequent de‐quenching, although no signal is produced by amplification of off‐concentrate https://www.facebook.com/NewTechFind/posts/pfbid0YPAzGvVz72mKah68ctQg3wP1JFbs9jq9dyoRfz9AkydHS5u7wshhDSMEEcCg7NQCl